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KMID : 1098420060140020092
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 2 p.92 ~ p.95
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Rapid Propagation of Pelagonium Inquinans Via Organogenesis from Mature Leaf Explants
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Hwang Sung-Jin
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Abstract
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A method for plant regeneration via organogenesis from Pelagonium inquinans leaf disc has been developed. Mature leaf explants were collected from field-grown plants and used for the induction of adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose plus plant growth regulators. Maximum shoot organogenesis, with 11.8{pm}1.5 shoots (98.6%) per leaf disc, was obtained with 2;mg/l N^6-benzyladenine (BA) and 0.5;mg/l {alpha}-naphthyleneacetic acid (NAA) in 30 days. For rooting, the in vitro proliferated and elongated shoots were excised into 1.5-2 cm in length microcutting, which were plated individually on an half-strength MS (1/2MS) medium supplemented with 2% (w/v) sucrose plus various concentrations of indole-3-butyric acid (IBA). Shoots rooted with a frequency of 100% following culture on 1/2MS medium containing 0.5;mg/l IBA.
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KEYWORD
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Pelagonium inquinans, mature leaf, organogenesis, plant regeneration
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